Dear Clement,
Hi,
Thanks for your comment and attention.
Best wishes
--- On Fri, 11/14/08, xtal-log-request from oat.bio.indiana.edu <xtal-log-request from oat.bio.indiana.edu> wrote:
From: xtal-log-request from oat.bio.indiana.edu <xtal-log-request from oat.bio.indiana.edu>
Subject: Xtal-log Digest, Vol 39, Issue 3
To: xtal-log from magpie.bio.indiana.edu
Date: Friday, November 14, 2008, 8:33 PM
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Today's Topics:
1. Re: I want to crystallize one protein with signal peptide on
N-terminal, Is it possible? (Clement Angkawidjaja)
2. I want to crystallize one protein with signal peptide on
N-terminal, Is it possible? (Mitra Kheirabadi)
----------------------------------------------------------------------
Message: 1
Date: Fri, 14 Nov 2008 11:44:57 +0900
From: "Clement Angkawidjaja"
<clement from bio.mls.eng.osaka-u.ac.jp>
Subject: [Protein-crystallography] Re: I want to crystallize one
protein with signal peptide on N-terminal, Is it possible?
To: <xtal-log from oat.bio.indiana.edu>
Message-ID: <BDD6507F8AFE4BF29175326FE32CD0D5 from Clement>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Hi Mitra,
The answer, like most answers about protein crystallization, is "It
depends."
The thing is, most proteins with N-terminal signal sequence are not
(completely) folded when the signal is intact. But since you mentioned that
yours is active with the signal, it MAY crystallize. However, I would
suggest that you perform crystallization screening using both the intact and
signal sequence-removed constructs. This way you can save a lot of time if
one of them does not crystallize well.
Regards,
Clement Angkawidjaja, Ph.D
Assistant professor
Laboratory of Molecular Biotechnology
Division of Advance Science and Biotechnology
GSE Common East 8F
2-1 Yamadaoka, Suita-shi, Osaka, 565-0871, Japan
Tel/fax: +81-6-6879-4580
----- Original Message -----
From: <xtal-log-request from oat.bio.indiana.edu>
To: <xtal-log from magpie.bio.indiana.edu>
Sent: Friday, November 14, 2008 2:03 AM
Subject: Xtal-log Digest, Vol 39, Issue 2
> Send Xtal-log mailing list submissions to
>xtal-log from net.bio.net>> To subscribe or unsubscribe via the World Wide Web, visit
>http://www.bio.net/biomail/listinfo/xtal-log> or, via email, send a message with subject or body 'help' to
>xtal-log-request from net.bio.net>> You can reach the person managing the list at
>xtal-log-owner from net.bio.net>> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Xtal-log digest..."
>>> Today's Topics:
>> 1. I want to crystallize one protein with signal peptide on
> N-terminal, Is it possible? (Mitra Kheirabadi)
>>> ----------------------------------------------------------------------
>> Message: 1
> Date: Wed, 12 Nov 2008 11:30:17 -0800 (PST)
> From: Mitra Kheirabadi <mitrakheirabadi from yahoo.com>
> Subject: [Protein-crystallography] I want to crystallize one protein
> with signal peptide on N-terminal, Is it possible?
> To: xtal-log from magpie.bio.indiana.edu> Message-ID: <140112.69149.qm from web36301.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>> Hi everybody
>> I wanna crystallize a 16 kDa protein with signal peptide on the N-terminl,
> is it correct? Protein activity is good with signal peptide but I
don't
> know if this signal peptide effect on 3D structure? Would you please guide
> me?
>> Best
>> Mitra
>>>>> ------------------------------
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>Xtal-log from net.bio.net>http://www.bio.net/biomail/listinfo/xtal-log>> End of Xtal-log Digest, Vol 39, Issue 2
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------------------------------
Message: 2
Date: Wed, 12 Nov 2008 11:30:17 -0800 (PST)
From: Mitra Kheirabadi <mitrakheirabadi from yahoo.com>
Subject: [Protein-crystallography] I want to crystallize one protein
with signal peptide on N-terminal, Is it possible?
To: xtal-log from magpie.bio.indiana.edu
Message-ID: <140112.69149.qm from web36301.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi everybody
I wanna crystallize a 16 kDa protein with signal peptide on the N-terminl, is
it correct? Protein activity is good with signal peptide but I don't
know if this signal peptide effect on 3D structure? Would you please guide me?
Best
Mitra
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