> Hi all
> Recently i got a mutant gene and showing higher solubility then wild
> type. we are assuming that our mutant preventing the aggregation of the
> protein.
> I would like to study the thermal and chemical protein aggregation.
> i would be happy If any one provide me detailed protocol to study this
> experiment.
Besides DSC, you can also do CD spectroscopy. Easier and needs smaller
amount of protein (10 mg for far-UV should be sufficient). Similar with Dr.
Engelbert Buxbaum's comment, you need to collaborate with someone with
appropriate instrument.
Clement
