From gerchman from research.haifa.ac.il Thu Feb 7 14:28:13 2008 From: gerchman from research.haifa.ac.il (Yoram Gerchman) Date: Thu Feb 7 18:00:43 2008 Subject: [Mycology] Acidic pH and fungal growth Message-ID: <1202412493.47ab5bcd993c9@webmail.haifa.ac.il> Greetings netters This is probably a silly question, but what would be the effect of acidic pH (like 3~4) on the growth of fungi such as Pandora sp.? Many thanks Yoram ------------------------------------------------------------------------ This message was sent using IMP, the Webmail Program of Haifa University From mycos from shaw.ca Thu Feb 7 20:36:31 2008 From: mycos from shaw.ca (mycos) Date: Thu Feb 7 23:34:03 2008 Subject: [Mycology] Acidic pH and fungal growth In-Reply-To: <1202412493.47ab5bcd993c9@webmail.haifa.ac.il> References: <1202412493.47ab5bcd993c9@webmail.haifa.ac.il> Message-ID: <47ABB21F.3010700@shaw.ca> As it happens, a Mor humus form is by-and-large the preferred soil type for most of the mycorrhizae. Not being a soil scientist myself I don't want to get too far ahead here, but I don't believe the term "Mycelial Mor" has a counterpart when it comes to Moder or Mull soils/humus forms. A Mor is of course more acidic than the other 2 broad classifications. A PH of 3 otoh is getting awfully acidic for most plants however. As for "Pandora"? Unless that's a new name for an old taxa, I don't believe I've ever heard of a macrofungus with that particular name. Yoram Gerchman wrote: > Greetings netters > This is probably a silly question, but what would be the effect of acidic pH > (like 3~4) on the growth of fungi such as Pandora sp.? > > Many thanks > Yoram > > ------------------------------------------------------------------------ > This message was sent using IMP, the Webmail Program of Haifa University > > _______________________________________________ > Mycology mailing list > Mycology@net.bio.net > http://www.bio.net/biomail/listinfo/mycology > From gerchman from research.haifa.ac.il Fri Feb 8 02:21:33 2008 From: gerchman from research.haifa.ac.il (Yoram Gerchman) Date: Fri Feb 8 13:32:17 2008 Subject: [Mycology] Acidic pH and fungal growth In-Reply-To: <47ABB21F.3010700@shaw.ca> References: <1202412493.47ab5bcd993c9@webmail.haifa.ac.il> <47ABB21F.3010700@shaw.ca> Message-ID: <1202455293.47ac02fd3ee8d@webmail.haifa.ac.il> Maybe I missed something trivial here Pandora, to the best of my knowledge, are entomopatogenic fungi beloinging to the Zygomycota. Thanks Yoram Quoting mycos : > As it happens, a Mor humus form is by-and-large the preferred > soil type for most of the mycorrhizae. Not being a soil > scientist myself I don't want to get too far ahead here, but I > don't believe the term "Mycelial Mor" has a counterpart when > it comes to Moder or Mull soils/humus forms. A Mor is of > course more acidic than the other 2 broad classifications. A > PH of 3 otoh is getting awfully acidic for most plants however. > > As for "Pandora"? Unless that's a new name for an old taxa, I > don't believe I've ever heard of a macrofungus with that > particular name. > > Yoram Gerchman wrote: > > Greetings netters > > This is probably a silly question, but what would be the effect of acidic > pH > > (like 3~4) on the growth of fungi such as Pandora sp.? > > > > Many thanks > > Yoram > > > > ------------------------------------------------------------------------ > > This message was sent using IMP, the Webmail Program of Haifa University > > > > _______________________________________________ > > Mycology mailing list > > Mycology@net.bio.net > > http://www.bio.net/biomail/listinfo/mycology > > > ------------------------------------------------------------------------ This message was sent using IMP, the Webmail Program of Haifa University From newmy51 from gmail.com Sat Feb 9 20:48:25 2008 From: newmy51 from gmail.com (Danny Newman) Date: Sat Feb 9 20:52:43 2008 Subject: [Mycology] Fluoride/Fluorine Hyperaccumulators Message-ID: <24a95ed90802091748h1f4ed7f3n3f832cef39d22798@mail.gmail.com> Stamets? Mycelium Running illustrates just a sampling of some of the heavy metal accumulator candidates in the fungal world, but it?s an admittedly incomplete list; a work in progress. Seeing as fluoride is an incredibly prolific poison -- present in just about everything from Prozac to potatoes -- and is very expensive to filter, I?m interested to find out if there is a fungus which could feasibly complement areas with mild to massive fluoride concentrations in the soil or water, and absorb or bind with the fluoride molecules through the mycelia. Some plants, such as Hydrocotyle umbellate L. (Water Pennywort), have been demonstrated as good fluoride accumulators, but a fungal match would have the advantages of both mycelium over roots (greater square area for absorption), and being an overall easier organism to maintain, among countless others. Like the heavy metal macrofungal accumulators, they?d need to be kicked as a food source, and I dare not think of selling the reconcentrated compound back to whoever it came from, but at least it?s out of the food chain. Any thoughts? From geis.pa from pg.com Mon Feb 11 07:40:31 2008 From: geis.pa from pg.com (Geis, Phil) Date: Mon Feb 11 13:49:52 2008 Subject: [Mycology] RE: Mycology Digest, Vol 32, Issue 3 In-Reply-To: <200802091705.m19H5vL19729@net.bio.net> References: <200802091705.m19H5vL19729@net.bio.net> Message-ID: <3B4A11B0E75E524AB9EFDF2BDC5CE4F8BD0C2C@bdc-emb005.na.pg.com> glwww.mst.dk/udgiv/publications/2001/87-7944-343-5/html/kap05_eng.htm pH In general Entomophthorales are tolerant of pH levels between 6 and 7 with an optimum of approximately 6.5 for the species investigated so far. At pH below 5 and above 8 growth is remarkably depressed (Gustafsson, 1965; Latg? et al. 1977; Dunphy & Nolan, 1979; Latg? & Sanglier, 1985; Robinson, 1986). For P. neoaphidis the fastest growth rate has been obtained between pH 6.0 and 7.0 (Gustafsson, 1965; Robinson, 1986). The optimal pH corresponds well with the pH usually found in insect haemolymph. Gustafsson (1965) concluded that P. neoaphidis has a relatively narrowly limited pH optimum compared with species from the genus Conidiobolus. -----Original Message----- From: mycology-bounces@oat.bio.indiana.edu [mailto:mycology-bounces@oat.bio.indiana.edu] On Behalf Of mycology-request@oat.bio.indiana.edu Sent: Saturday, February 09, 2008 12:06 PM To: mycology@magpie.bio.indiana.edu Subject: Mycology Digest, Vol 32, Issue 3 Send Mycology mailing list submissions to mycology@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/mycology or, via email, send a message with subject or body 'help' to mycology-request@net.bio.net You can reach the person managing the list at mycology-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Mycology digest..." Today's Topics: 1. Re: Acidic pH and fungal growth (Yoram Gerchman) ---------------------------------------------------------------------- Message: 1 Date: Fri, 8 Feb 2008 09:21:33 +0200 From: Yoram Gerchman Subject: Re: [Mycology] Acidic pH and fungal growth To: mycos Cc: mycology@magpie.bio.indiana.edu Message-ID: <1202455293.47ac02fd3ee8d@webmail.haifa.ac.il> Content-Type: text/plain; charset=ISO-8859-1 Maybe I missed something trivial here Pandora, to the best of my knowledge, are entomopatogenic fungi beloinging to the Zygomycota. Thanks Yoram Quoting mycos : > As it happens, a Mor humus form is by-and-large the preferred > soil type for most of the mycorrhizae. Not being a soil > scientist myself I don't want to get too far ahead here, but I > don't believe the term "Mycelial Mor" has a counterpart when > it comes to Moder or Mull soils/humus forms. A Mor is of > course more acidic than the other 2 broad classifications. A > PH of 3 otoh is getting awfully acidic for most plants however. > > As for "Pandora"? Unless that's a new name for an old taxa, I > don't believe I've ever heard of a macrofungus with that > particular name. > > Yoram Gerchman wrote: > > Greetings netters > > This is probably a silly question, but what would be the effect of acidic > pH > > (like 3~4) on the growth of fungi such as Pandora sp.? > > > > Many thanks > > Yoram > > > > ------------------------------------------------------------------------ > > This message was sent using IMP, the Webmail Program of Haifa University > > > > _______________________________________________ > > Mycology mailing list > > Mycology@net.bio.net > > http://www.bio.net/biomail/listinfo/mycology > > > ------------------------------------------------------------------------ This message was sent using IMP, the Webmail Program of Haifa University ------------------------------ _______________________________________________ Mycology mailing list Mycology@net.bio.net http://www.bio.net/biomail/listinfo/mycology End of Mycology Digest, Vol 32, Issue 3 *************************************** From anne from eaglehill.us Thu Feb 14 15:57:28 2008 From: anne from eaglehill.us (anne@eaglehill.us) Date: Thu Feb 14 19:35:26 2008 Subject: [Mycology] Eagle Hill Bryology seminars - Maine Message-ID: ANNOUNCING ... 2008 Bryology Seminars at the Humboldt Institute on the coast of Maine! June 29 - July 5 Calicioid Lichens and Fungi: Identification, Ecology, and Role in Assessing Forest Continuity Steven Selva July 6 - 12 ..... Lichenicolous Fungi: North America's Second Sick Lichen Masterclass David L. Hawksworth August 3 - 9 .... Polypores, Tooth Fungi, and Crust Fungi Thomas Volk and Sean Aug 31 - Sept 6 . Mycology for Naturalists: Diversity, Biology, and Ecology of Fungi and Fungal-like Organisms David Porter Sept 28 - Oct 4 . Advanced Mycology: Freshwater and Marine Ascomycetes Carol Ann Shearer Descriptions of seminars may be found at http://www.eaglehill.us/mssemdes.html Information on lodging options, meals, and costs may be found at http://www.eaglehill.us/mapinfo.html There is a printable and online application form at http://www.eaglehill.us/mapweb.html http://www.eaglehill.us/mapprn.html Syllabi are available for these and many other fine natural history training seminars on diverse topics. For more information, please contact the Humboldt Institute, PO Box 9, Steuben, ME 04680-0009. 207-546-2821. Fax 207-546-3042 E-mail - mailto:office@eaglehill.us Online general information may be found at http://www.eaglehill.us NATURAL HISTORY SEMINARS In support of field biologists, modern field naturalists, and students of the natural history sciences, Eagle Hill offers specialty seminars and workshops at different ecological scales for those who are interested in understanding, addressing, and solving complex ecological questions. Seminars topics range from watershed level subjects, and subjects in classical ecology, to highly specialized seminars in advanced biology, taxonomy, and ecological restoration. Eagle Hill has long been recognized as offering hard-to-find seminars and workshops which provide important opportunities for training and meeting others who are likewise dedicated to the natural history sciences. Eagle Hill field seminars are of special interest because they focus on the natural history of one of North America's most spectacular and pristine natural areas, the coast of eastern Maine from Acadia National Park to Petit Manan National Wildlife Refuge and beyond. Most seminars combine field studies with follow-up lab studies and a review of the literature. Additional information is provided in lectures, slide presentations, and discussions. Seminars are primarily taught for people who already have a reasonable background in a seminar program or in related subjects, or who are keenly interested in learning about a new subject. Prior discussions of personal study objectives are welcome. End of file... Thank you. From FloridaMycology from cs.com Tue Feb 19 21:44:04 2008 From: FloridaMycology from cs.com (FloridaMycology@cs.com) Date: Wed Feb 20 12:54:11 2008 Subject: [Mycology] #23 "TEONANACATL" Mushroom Journal Posted Up Message-ID: <213707d0-8042-41ea-ba2b-6587d839b9ae@41g2000hsc.googlegroups.com> The #23 "TEONANACATL" February 2008 Mushroom Journal is now posted up at: http://www.mushroomsfmrc.com/gpage5.html For free inspection and download. Here is a partial list of this current issue's articles. C O N T E N T S #23 "TEONANACATL" February 2008 Subscription and Other Journal Information............................................01 Mail Call.................................................................................................. 02 Converting Ibotenic Acid Into Muscimol.............................................02 Liberty Caps Talk, MushroomJohn....................................................02 Are there any Psilocybes that are "DEADLY?"..................................03 Recovery of Mushroom Mycelium From Liquid Culture Media.......... 03 Medicinal Benefits From Psilocybe Mushrooms................................04 Laws On Ordering & Possession Of Mushroom Spores....................05 Dr. Karl Speaks on Medicinal Psilocybes...........................................05 More On Mushroom Spore Laws.......................................................06 Galerina autumnalis or G. marginata?...............................................06 Drying Out Mushrooms........................................................................... 07 Oops......Illegal Mushroom Operation.....................................................07 Can You Really Convert Ibotenic Acid Into Muscimol With Heat?......... 08 PSILOCYBIAN GYMNOPILUS MUSHROOMS IN THE WORLD..........08 Lambs Bread.......................................................................................... 13 The Duna Mushroom and the Fang Bwiti Cult of Gabon.......................14 Why Mushroom Mycelium Degenerates and Dies Off...........................14 What Are The State Laws On Mushrooms and Their Spores & Cultures?.15 Are Mushroom Laws Too Harsh???.......................................................16 "The Magic PSTMP Compound"............................................................ 16 C L A S S I F I E D.................................................................................. 23 $2.00 Mushroom Spore Print Sample Back Up Again............................23 Spore Print sample free and affixed inside Psilocybe cubensis.............24 From hiromoto from aloha.net Fri Feb 22 18:54:06 2008 From: hiromoto from aloha.net (Bryan Hiromoto) Date: Fri Feb 22 23:42:04 2008 Subject: [Mycology] Truffles Message-ID: <179D47D7-7A05-4822-B978-5B81A0ED1B34@aloha.net> Hello, Does anyone have a description of Truffle mycelium in culture? Regards, Bryan Hiromoto From fdepuydt from gmail.com Sun Feb 24 10:12:53 2008 From: fdepuydt from gmail.com (Frederick Depuydt) Date: Sun Feb 24 14:31:34 2008 Subject: [Mycology] Re: Mycology Digest, Vol 29, Issue 8 In-Reply-To: <200711291704.lATH44Y26214@net.bio.net> References: <200711291704.lATH44Y26214@net.bio.net> Message-ID: <4b8d554e0802240712j3e44a0b3r53e0199bbf480aac@mail.gmail.com> > While a student at Oregon State University, I was informed that Helen > Gilkey had cultivated several species of Choleorhiza (sp?) orchids by > first growing Rhizopogon vinicolor (a very common hypogeous fungi > which the orchids live off of) first in association with Douglas-fir > seedlings. She was the only preson I know of who has been successful > at such cultivation. > > It is illegal in California and Oregon to possess even a single flower > of these orchids. The fine, as I recall, used to be $500 per flower, > and a single stem of these orchids may have 40-200 flowers/buds/seed > pods on it. So possession of a stem if you are caught could net you a > rather hefty fine. OTOH, very few people know these are orchids > anyway... > > Rhizopogon vinicolor is relatively easy to introduce to Douglas-fir. A > simple slurry of sporocarps sprayed 2-4 times on seedling trees will > ensure nearly 95% colonization of the rootlets if done during the > spring. Each slurry application should be done after 2-3 weeks > intervals, and preferably during a rain so spores can be washed into > the soil near the seedling tree rootlets. Spores not in close > proximity to rootlets do not appear to germinate. > > Since R. vinicolor is mostly spores, a single average sporocarp of 1 > inch diameter will inoculate around a million seedling trees, or > enough to reforest several square miles at 700 trees per acre. > > Daniel B. Wheeler Hello Daniel, I haven't got a clue why this happened but I only now found back your answer, my apologies for this belated reply. I think you are talking about the genus Coralorhiza, right ? There are indeed a number of mycorrhiza that have a treesome relationship, one of the fungi which is responsible for keeping Calypso bulbosa alive is also suspected of being associated with the roots of trees. This limits the cultivation of these orchids to the protocorm stage, extended culture would rely on a habitat with shrubs or trees that have their roots innoculated. The aclorophylous (such as those in Coralorhiza) will probably never be cultivated, but the specificity of other genera needs to be tested on these fungi. Thanks for providing a piece of the puzzle. Frederick From falconem from univmail.cis.mcmaster.ca Fri Feb 29 18:27:28 2008 From: falconem from univmail.cis.mcmaster.ca (Mina Falcone) Date: Fri Feb 29 18:53:32 2008 Subject: [Mycology] gene deletion via PCR Message-ID: I am attempting to create a deletion mutant in S. cerevisiae using PCR and inserting his3 in place of my gene of interest as the selectable marker. My confirmation PCR reactions (done by colony PCR) used two sets of primers: one set consisted of an upstream primer 200bp from the ORF plus a downstream primer about 600bp into the selectable marker (his3), thus giving a PCR product of approximately 800bp if the deletion/insertion was successful. The other set of primers are 200 and 400 bp, respectively, upstream and downstream from the original ORF. The wildtype gives a PCR fragment of 1200bp and the insertion/deletion should give a fragment of approx. 2000bp. My problem is that in 2 out of 35 colonies that I picked from the -his selective plates the first set of primers gives the correct size of product (800bp) indicating that the insertion is there; but, the second set of primers gives a dominant band at 1200bp (the size of the WT gene that I'm trying to replace) and a very faint band that is approx. the size expected for the selectable marker. Has anyone ever experienced this before? I am puzzled by it. Does it mean that there is only a partial insertion? How can this be if the colony originated from a single cell? Are there revertants within the colony? Should I further streak out these quasi positives or should I screen more colonies? Any advice would be greatly appreciated. Mina Mina Falcone Research Assistant McMaster University Department of Biochemistry and Biomedical Sciences Health Sciences Centre Room 4H41 1200 Main Street West Hamilton, ON L8N 3Z5 905-525-9140 ext 22050 falconem@mcmaster.ca