"rOHITASH jAMWAL" <rohitash13 from gmail.com> wrote in message
news:mailman.180.1211468470.3533.microbio from net.bio.net...
> Hello folks
> I am trying to extract my compounds from TLC plate(Sillica gel)...I have
> used ethanoloc extracts for assay.
>> Could anyone please guide me how to isolate active compounds from sillica
> gel.
>> Rohitash
>rohitash13 from gmail.com
Hi Rohitash
What type or class of compound do you think you might be extracting ?
Is an ethanolic extractant the best choice ?
Look to the composition of the mobile phase which produced the target band
in the first place. The chemical composition of that should indicate the
nature of a suitable extractant for you
Also how do you know that your current approach has been unsuccesfull. ?
What you mean by an active compound ?
Bets N10 ;)
