Aawara Chowdhury <aawara from pontiff-playground.org> wrote in
news:vKbUl.117450$HZ1.10056 from newsfe15.iad:
> In <Xns9C1ADA06B9F68ikkezelf from 216.151.153.60>,
> Han <nobody from nospam.not> wrote:
>>>> Details? What antybiotics - the usual anti-bacterial ones or the
>>> selective drug? No experience with lentivirus but can tell about
>>> transfections: if the former, then the claims are simply not true -
>>> transfections work fine with the pen/strep or gentamicin; if the
>>> latter, then the reason for not working should be obvious.
>>>>>> DK
>>>>> It was the usual pen/strep. Thanks for the quick reply. I'll have
>> to needle my colleagues. They stated that it was well-known it
>> didn't work, but couldn't explain why.
>>>> Agree 100% with Dima. We do lentiviral/retroviral infections all the
> time, in media containing antibiotics. Now anti-fungals are another
> matter. Many of them impact the infectivity of enveloped viruses that
> contain cholesterol rich lipid membranes - like retroviruses and
> lentiviruses.
>> AC
>Thanks, Aawara. I can easily see antifungals interfering with membrane
trafficking.
--
Best regards
Han
email address is invalid
