I have it on good authority from a labmate that sucrose-gradients are
a great way to check for protein localisation. I gather it involves a
long centrifuge cycle but yields very reliable results.
On 10 Jun 2009, at 11:06, Jinxiang Wang <jinxwang from scau.edu.cn> wrote:
> Dear Colleaguses,
>> I am going to localize a protein which is from soybean.Bioinformatic
> analysis indicates it is maybe licalized in ER membrane.Initially I
> would likt to fuse my protein to GFP and check its
> localization.Alternatively I can employe antibody against my protein
> to
> verify its localization. Certainly the later technique is
> time-consuming. Would you kindly give me your suggestions related to
> special marker dyes for ER or ER-special marker proteins? In addtion I
> know some special dyes and antibody for ER-special marker proteins are
> commercially availabe. I look foward to comments or ideas of experts
> to
> guide me to select better dyes or marker protein Ab? Many thanks in
> advance.
>> Best regards.
>> Jinxiang
>>> --
> ----------------------------------------------------
> Jinxiang Wang Ph.D
>> College of Resources and Environment Science
> Root Biology Center
> South China Agricultural University
> Guangzhou 510642, P.R.China
>> Email: jinxwang from scau.edu.cn> Tel: +86-20-85280156 (O)
> Fax: +86-20-85281829
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