Dear Colleaguses,
I am going to localize a protein which is from soybean.Bioinformatic
analysis indicates it is maybe licalized in ER membrane.Initially I
would likt to fuse my protein to GFP and check its
localization.Alternatively I can employe antibody against my protein to
verify its localization. Certainly the later technique is
time-consuming. Would you kindly give me your suggestions related to
special marker dyes for ER or ER-special marker proteins? In addtion I
know some special dyes and antibody for ER-special marker proteins are
commercially availabe. I look foward to comments or ideas of experts to
guide me to select better dyes or marker protein Ab? Many thanks in advance.
Best regards.
Jinxiang
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Jinxiang Wang Ph.D
College of Resources and Environment Science
Root Biology Center
South China Agricultural University
Guangzhou 510642, P.R.China
Email: jinxwang from scau.edu.cn
Tel: +86-20-85280156 (O)
Fax: +86-20-85281829