Use a buffer (10mM Tris-EDTA, pH 6.5 to 7.2, or 50mM phosphate buffer
should work) and add glycerol to 35% (as high as 50%). Store in a -20C
freezer (no auto defrost).
Cellulase can also be stores freeze dried after the appropriate dialysis
against distilled water containing 1mM DTT.
>> I have purified cellulase enzyme through His tage and interested to store
> the purified protein. I shall be glad to know the recepi for stable storge
> of purified enzyme.
>>>> _______________________________________________
> Methods mailing list
>Methods from net.bio.net>http://www.bio.net/biomail/listinfo/methods>
--
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003
