neda akbari <depbioakbari from yahoo.com> wrote in
news:mailman.1614.1233164292.29717.methods from net.bio.net:
> i want to isolate an enzyme which bound to cell membrane. i used
> Tris-buffer with triton X-100 ( 0.5 %) pH= 8 by sonication but most of
> them didnt isolated by comparing with SDS-PAGE.can you help me? thank
> you in advance
>It's possible the enzyme isn't soluble in Triton, or that it requires more
than 0.5%. Many membrane proteins are insoluble in Triton. One of the
ways to prepare cytoskeletal proteins is to remove the Titon-soluble
proteins, and then study what's left.
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Best regards
Han
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