We regularly purify proteins on RPHPLC C18 column. I have never heard of any protein getting pptd. with ACN. I suggest you to go ahed with your purification!
All the best!
On Thu, 22 Jan 2009 Mouldi SAIDI wrote :
>Dear all,
>I would like to ask if the separation of casein using HPLC, C18 RP and acetonitrile/water TFA is possible.
>I'm afraid that acetonitrile precipitates proteins?
>Best regards
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Shifali Chatrath
Graduate Student
Protein science Lab
Dept. of Biological sciences
National University of Singapore
Singapore
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