Hi Everyone,
I need a vector that is membrane specific for my control cell line. When I use the HEK293 cell line (control - does not have receptor in membrane or else where), after transfection I have a diffuse cytoplasmic EGFP+. When I use the HTC cell line (experimental - receptor is present in the membrane), I have a specific membrane EGFP+ product. I am currently using pIRES2-EGFP. Any help is appreciated. Thanks, Shar
