Dear experts,
I am relatively new in the Real-time PCR world. I am about to order a
bunch of hydrolysis probes quenched with the BHQ.
The company states exclusively that they can do 3' - BHQ labeling but
is this also mean that the 3'-OH will be blocked by the quencher
against extension or I must order 3' phosphorylation in addition.
Any suggestions will be most welcome...
Thanks
Ivan