> Yet some refold very easily. It's always, always worth a shot.
> Easy to do, nothing to lose.
> I slightly disagree: For proteins where NO rock solid functional test is
> available for proper validation of refolding success, the risk of ending up
> with a heterogeneous population of partly refolded and partly misfolded
> protein is significantly increased by refolding (vs. e.g. soluble expression
> under cold-shock conditions) making it completely useless for most (ensemble
> based) biophysical and structure biology assays.
cheers,
>> David
>> _______________________________________________
> Methods mailing list
>Methods from net.bio.net>http://www.bio.net/biomail/listinfo/methods>
--
Science is what happens while we are making other plans (~John Lennon)
