Dear all,
I have to generate several chimeric proteins where I will have to
insert about 50 aa residues of one protein in the middle of another. I
was searching through the archives and found the Emerman, Deminie
protocol ( J.Virol , July 1993 p.6499-6506). Basically one has to do
four PCR reactions followed by a restriction digest and ligation. Is
this still the most efficient way to generate chimeric proteins where
the insertion size is relatively big ? Any help will be really
appreciated.
Best regards,
AB.
