Dear experts, I am doing a co-immunoprecipitation experiment. I pull down
protein X with a mAb and detect co IP of protein Y with pAb. protein Y is
heat sensitive and if I heat (~70C) the sample to elute immune complex, I
can not detect protein Y. without heating, protein-G beads do not let the
IgG chains loose, at least. Are there other ways to elute immune complex
without heating? of course low pH is one obvious strategy that I have not
tried yet. thank you in advance.
AK
