Hi guys,
I've recently had some problems getting RNA for some qPCR but my main
problem is that I run a standard curve with all my sets of primers to make
sure I can compare the efficiency of the reaction properly. With my current
RNA yield I don't have enough to run a standard curve. There are
differences of opinion in our lab, one person says always run a standard
curve to make sure the efficiency is the comparable and one person says you
don't need a standard curve just use the controls to compare levels and do
Delta:Delta CT.
Any thoughts?
Cheers
Yvonne
