and this!
http://www.protocol-online.org/biology-forums/posts/25380.html
i have done triple transient transfections..but yes..cell death is little
more than single/double. But i seed more cells than i usually for
single/double..and it seems to work..
plasmid expression can be altered depending on promoter sequence..especially
multiple plasmid with different promoters..i try keeping them constant
(CMV-all CMV, that way)
best,
sudheendra.
On Thu, May 8, 2008 at 8:19 PM, Sudheendra Rao N R <sudhee26 from gmail.com>
wrote:
> Hey, look in to some of these
>http://www.springerlink.com/content/u3531825942t18v5/>>http://www.bio.net/hypermail/methds-reagnts/2001-September/090500.html>>http://www.bio.net/hypermail/methds-reagnts/2001-September/090499.html>> best,
> sudheendra.
>> On Mon, May 5, 2008 at 11:49 PM, Wendy Grus <wgrus from umich.edu> wrote:
>> > Hi all-
> > I am transfecting some HEK 293 T cells to transiently express a few
> > different genes. Some of these genes are on pcDNA3.1 vectors while some
> > are
> > on pCI vectors. Will having them on different vectors affect their
> > expression levels? Will I have to play with the concentrations of each?
> > Should I put all the genes in one vector (singly)?
> >
> > Thanks,
> > Wendy Grus
> > _______________________________________________
> > Methods mailing list
> > Methods from net.bio.net> > http://www.bio.net/biomail/listinfo/methods> >
>>>> --
> Think before agree
> Think before you nod
> but STOP thinking
> and You Are God
--
Think before agree
Think before you nod
but STOP thinking
and You Are God
