Hi all-
I am transfecting some HEK 293 T cells to transiently express a few
different genes. Some of these genes are on pcDNA3.1 vectors while some are
on pCI vectors. Will having them on different vectors affect their
expression levels? Will I have to play with the concentrations of each?
Should I put all the genes in one vector (singly)?
Thanks,
Wendy Grus