nick from biteisizebio wrote about these "safe" stains recently, also
calculating cost per 100ml:
http://bitesizebio.com/2008/03/03/ethidium-bromide-the-alternatives/
he writes that methylene blue is "Post strain only, in 0.025% (w/v)
methylene blue in water." and according to him only bands above 500ng
are visible.
methylene blue is also part of those "run your DNA at home" instructions:
http://www.scq.ubc.ca/the-macgyver-project-genomic-dna-extraction-and-gel-electrophoresis-experiments-using-everyday-materials/
cheers
Max
2008/5/4 Gita Bangera <gbangera from bcc.ctc.edu>:
> Hello,
>>>> Has anyone tried adding methylene blue to their DNA sample before
> running it on the gel? Since methylene blue stains DNA, I am wondering
> if that would work. I am not interested in cutting out the bands from
> this gel or using the DNA in any way. I just want my students to be able
> to visualize the DNA on the gel and I really don't want to have to deal
> with EtBr.
>>>> Thank you for your time.
>>>> Gita
>>>> Gita Bangera Ph.D.
>> Instructor, Life Sciences (Biology)
>> Bellevue Community College
>> Tel: 425-564-4031
>> Fax: 425-564-4125
>>>> _______________________________________________
> Methods mailing list
>Methods from net.bio.net>http://www.bio.net/biomail/listinfo/methods>
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Maximilian Haeussler,
tel: +33 6 12 82 76 16
