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Digestion Problem

Carla Beukes via methods%40net.bio.net (by carla.beukes from gmail.com)
Sat Mar 15 03:37:03 EST 2008

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I am currently working with the Beta 2 adrenergic receptor polymorphims,
Arg16Gly and Glu27Gly.  The PCR seems to be working fine and I get product
but after I have digested with the enzymes NCoI or BbvI nothing shows up on
the gel.  It is completely blank. Any suggestions as to what I may be doing
wrong?


Carla Beukes
(carla.beukes from gmail.com)

Previous message: Dam and Dcm methylation problem
Next message: colony PCR
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