colony PCR

[Sunil Dalvi]

I am in need of colony PCR method . I got the positive colonies for Agro but
not getting the band for insert. I got the band for positive control in the
same reaction.
where would be the problem

-- 
S.G.Dalvi,
Vasantdada Sugar Institute,Pune
Manjari bk, India
http://www.vsisugar.com/