Maurizio Affer <borderline86 from yahoo.com> wrote:
>I induce at an OD of 0.8 with IPTG final of 0.1mM for
>2 hours.
This is a quite short time after induction and also a very small
concentration for the IPTG. We ususally induce for 3-5 hours with 1mM
IPTG final using pGEX and BL21.
Christian
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X-no-Sig: yes
