diana_barbosa from sapo.pt wrote:
> Hello,
>> I'm having some trouble with the solubilization of proteins contained in
> a lipidic secretion we're studying (using 2DE).
> To overcome this problem, I'm preparing a series of trials with
> different lysis buffers, prepared with detergents
> alternative/complementary to CHAPS.
> One of the alternatives mentioned in the literature is Triton X-100, but
> in some papers it appears that this detergent doesn't help solubilize
> hydrophobic proteins but rather precipitates them, creating a 2
> fractions (hydrophilic and hydrophobic proteins). Is this true or am I
> misinterpreting?
>> Any help will be very welcome.
> Thank you,
>> Diana
>I think you mean Triton X-114, which is used for cloud point assays.
http://www.ncbi.nlm.nih.gov/pubmed/6257680?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
