Good question! I think long time ago ... in the last century ... Maniatis
et al.'s Lab Manual described the specificity of these enzymes. I have
not really used dNTP for phosphoryltion, so I don't know how efficient it
would be. Structurally the two are similar enough.
> Hi all,
>> have kinda silly question: Can T4PNK use dATP instead of rATP to
> phosphorilate DNA?
> Thanks ,
> Peter
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--
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003
