how about using ultrafiltration devices sold by amicon (part of millipore
now). 10 fold concentration is easily achieved by these. one example is here
http://www.millipore.com/catalogue/item/42423
AK
"kamalaker nasani" <agbiok4 from gmail.com> wrote in message
news:mailman.405.1213190547.3533.methods from net.bio.net...
> ---------- Forwarded message ----------
> From: kamalaker nasani <agbiok4 from gmail.com>
> Date: Wed, Jun 11, 2008 at 6:20 PM
> Subject: Protein work
> To: methods from net.bio.net, Plant Tissue Culture <PLANT-TC from lists.umn.edu>
>>> Good morning,
>>> I need a protocol to enrich or to concentrate protein of specific KD from
> plant material.
>> Details: I need to concentrate 10 times more of trasgene protein from
> seed
> powder. The gene is Cry1Ac.
>> If anybody having any material related to this please share with me.
>>>> --
> URS
> Kamalaker Nasani
>>>> "The illiterate of the 21st Century will not be those
> who cannot read or write. The illiterate will be those
> who cannot learn, unlearn and relearn"
> -George Bernard Shaw
>>>> --
> URS
> Kamalaker Nasani
>>>> "The illiterate of the 21st Century will not be those
> who cannot read or write. The illiterate will be those
> who cannot learn, unlearn and relearn"
> -George Bernard Shaw
