Peter Ellis wrote:
> TC wrote:
>> Hello,
>>>> I am trying to crosslink DNA to nylon membrane for southern blots.
>> Besides
>> traditional UV crosslinking (may induce DNA damage) and baking (causes
>> DNA
>> to denature), are there any other way to crosslink DNA onto nylon
>> memberanes? For my purpose, I hope minimize DNA damage and keep that
>> DNA in
>> native condition for the Southern blots.
>> Why do you want your DNA in native condition? Unless you denature it,
> it won't be accessible for your probe to bid to and you will have
> reduced signal.
>Not to mention that the type of DNA damage induced by UV does not
inhibit hybridization efficiency one bit.
Whoops, I mentioned it.
UV crosslink the nylon filter, bake the nitrocellulose filter. That's
how it's done.
