"TC" <tracyscience from gmail.com> wrote in message
news:mailman.777.1216254116.3533.methods from net.bio.net...
> Hello,
>> I am trying to crosslink DNA to nylon membrane for southern blots.
> Besides
> traditional UV crosslinking (may induce DNA damage) and baking (causes DNA
> to denature), are there any other way to crosslink DNA onto nylon
> memberanes? For my purpose, I hope minimize DNA damage and keep that DNA
> in
> native condition for the Southern blots.
>> THANKS~!
>> Tracy
Several positively charged membranes claimed to be ok for use without
conventional crosslinking. Personally I always UV-crosslink out of habit.
I doubt the damage on immobilised DNA is going to give you any trouble, and
I doubt immobilised DNA on a dry membrane is going to denature with heat...
and besides, for successful hybridisation you want the DNA to be denatured.
You may try a positively charged membrane to bypass heating/UV.
Out of curiosity, what are you trying to do?
Jose
