Hi Tracy,
I think DNA binds pretty strongly to positively charged membranes. I seem to
recall several occasions where, using charged membranes, crosslinking was
omitted but didn't seem to make any discernable difference...
HTH,
- Rory
> -----Original Message-----
> From: methods-bounces from oat.bio.indiana.edu> [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of TC
> Sent: 17 July 2008 08:40
> To: methods from magpie.bio.indiana.edu> Subject: DNA crosslinking on nylon memberane
>> Hello,
>> I am trying to crosslink DNA to nylon membrane for southern
> blots. Besides traditional UV crosslinking (may induce DNA
> damage) and baking (causes DNA to denature), are there any
> other way to crosslink DNA onto nylon memberanes? For my
> purpose, I hope minimize DNA damage and keep that DNA in
> native condition for the Southern blots.
>> THANKS~!
>> Tracy
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