On Sun, 13 Jul 2008, DK wrote:
>>"Zhong Silin" <stxsz1 from nottingham.ac.uk> wrote:
>>>> How can we detect alternative splicing in a living cell?
>>>> I assume that one way is to insert a fluorescent/epitope tag to a specific
>> region of that protein, which is present/absent in its splice variants.
>>>> Any suggestion please?
>> Why not Northern?
A) Because he wants to look at protein levels, not RNA levels
B) Because he wants to do it in living cells
I don't see the necessity for incorporating a reporter tag into the gene -
would it not be simpler to raise an antibody specifically to the variant
splice isoform? Introducing a tag would seem to run a large risk of
altering the function of the tagged exon.
Peter
