In <sBSxj.3$DO4.0 from newsfe02.lga>,
DK <dk from no.email.thankstospam.net> wrote:
>>There is a relatively new alternative to GFP for fluorescent tagging of
>>proteins in live cells in the shape of the FlAsH/ReAsH system:
>>>>http://www.invitrogen.com/content.cfm?pageid=11922>>>>Basically, you put a magic tag on your protein,
>> The magic tag with two exposed Cys is a subject to many
> crosslinking artifacts. Probably not a problem for most
> cytoplasmic proteins (reducing environment) but must
> be a huge problem for anything that goes through secretion
> pathway.
Any thoughts on the SNAP tag?
http://www.covalys.com/products/snap-cell-labeling-in-cells.html
I've been considering purchasing it.
AC
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