Hey Wang,
It looks like lipofectamine can have cytotoxicity depending on the cell line
and also what it is combined with.
one of the links is this.
http://www.protocol-online.org/biology-forums/posts/21720.html
Domic acid is speculated to be causing apoptosis in mouse cerebellum, which
can be reduced in a glutathione dependent manner.
So combine Domic acid and glutathione inhibitor and inject stereotaxically..
http://toxsci.oxfordjournals.org/cgi/content/abstract/100/2/433
i guess there will be better methods than this.
Sudheendra.
On Thu, Feb 21, 2008 at 10:37 AM, WANG XF <wxfhome from gmail.com> wrote:
> Thanks a lot for your information!
>> I found that transfection with liposome could lead to apoptosis of culture
> cells, but I did not find papers about it. Did you find some published
> papers about this? I am afraid that this will not be accepted by the
> reviewers when publish papers.
>> Regards.
>>> On 2/21/08, Sudheendra Rao N R <sudhee26 from gmail.com> wrote:
> >
> > How about using Lipofectamine?? stereotaxically injecting wherever you
> > want??
> > in culture it surely causes apoptosis.
> > i guess a cheaper alternative would be better anyway :)
> >
> > Sudheendra.
> >
> >
> >
> >
>
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