In <3kIUk.8581$ev2.3155 from newsfe12.iad>,
DK <dk from no.email.thankstospam.net> wrote:
> To the OP, I would suggest artificial coiled coils. Basically, a pair of
> 21 aa peptides used as interacting tags. One is very, very basic,
> another is very, very acidic - both conforming to the canonical coiled
> coil heptade repeat, so the only coiled coil formed is a heterodimer.
> I don't have a ref. but have a xerox somewhere at work. The affinity
> of such pair is subnanomolar.
I've used a modified version of the GCN4 leucine zipper for this purpose.
Sadly, I don't have the reference handy - I am at home, but this worked
very well to induce dimerization. If it helps any, the paper that I got
the sequence from was from Peter Kim's lab, and published in Biochemistry.
AC
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