Allison ha scritto:
> Fulvio Celsi wrote:
>> Dear All
>> I'm looking for suggestions on CoImmunoPrecipitation.... The protein
>> I'm interested in CoIP has a MW of 75Kd, so any time I made the
>> experiment, on the CoIP lane I saw a smear from the Igg (around 60kd
>> if I know well). How can I reduce this effect? I cannot buy another
>> Ab (from a different species) thing that I suppose would help...can I
>> cross-link ab to the protein-A beads? if yes...how??
>> Thanks a lot in advance
>> Fulvio
>>>> Could you run a non-reducing gel? Then the IgG would run at 150K vs
> 75K for your protein (assuming the latter is not subunits as well).
> Allison
ehm..unfortunatly not...the whole protein is bigger (tetramer)...but
good idea...
thanks!
Fulvio
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--
Fulvio Celsi
BsC,PhD
Dept. of Experimental and Diagnostic Medicine
Section of General Pathology
University of Ferrara
Via Borsari 46
44100 Ferrara
Italy
Mobile: +393286489131
http://salvarelaricerca.blogspot.com/
