Fulvio Celsi wrote:
> Dear All
> I'm looking for suggestions on CoImmunoPrecipitation.... The protein
> I'm interested in CoIP has a MW of 75Kd, so any time I made the
> experiment, on the CoIP lane I saw a smear from the Igg (around 60kd if
> I know well). How can I reduce this effect? I cannot buy another Ab
> (from a different species) thing that I suppose would help...can I
> cross-link ab to the protein-A beads? if yes...how??
> Thanks a lot in advance
> Fulvio
>
Could you run a non-reducing gel? Then the IgG would run at 150K vs 75K
for your protein (assuming the latter is not subunits as well).
Allison
