I guess you can try using NanoDrop as it should measure very small
quantity of primers..on agarose gel usually primers runs as dimers and
this gives a very "fuzzy" band difficult to quantify
good luck!
(and..redissolving primers and making another working solution?? :D )
TRAN HO QUANG ha scritto:
> Does anyone tell me how can measure primer concentration (working solution)
>> I am facing problems: the PCR reactions are not consistent. It is suggested that primer concentrations are not exact as it is 10 uM (working solution). The questions is can i use agarose or NanoDrop to check primer concentration?.
>>>> Any suggestions are warmly welcome!
>> Digifellow
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Fulvio Celsi
BsC,PhD
Dept. of Experimental and Diagnostic Medicine
Section of General Pathology
University of Ferrara
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Italy
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