how about trying to soak the gel and plates in buffer for seperation
2008/12/8 Nicosnake <chamosilencio from yahoo.com>:
>> HI everyone, i've been having this problem whit my acrylamide gels: after
> acrylamide is polymerized between the two glass plates it's really hard to
> separate both plates and after a long effort i can take them appart only to
> realize that the gel has sticked to BOTH plates, the gel is actually cut in
> half. I treat surfaces of both plates, one with bind silane (so the
> acrylamide sticks to this one) and the other with Sigmacote to prevent the
> gel from sticking. But still the gel sticks to both surfaces and after i
> separate the glases it's useless. Has anyonehad this kind of trouble?? ANy
> suggestion is accepted.
> --
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