hi all,
I recently did a nuclear protein isolation from cultured cells. After
western blot I can see my protein in nuclear and cytoplasmic fractions in
equal concentration.
So just to check the if there is any contamination of cytoplasmic protein in
my nuclear fraction, I am interested to check them aganist an antibody. This
antibody should be against a specific nuclear protein and should be present
in every cell.
I would appreciate any help in this case.
If someone can suggest any antibody that can detect only a cytoplasmic
protein also.
If any one has a good established protocol for nuclear protein isolation
please send me the link.
thanking you for ur help
--
Itisam Sarangi
