From pradeepiyer from heterodrugs.com Mon Oct 19 23:26:25 2009 From: pradeepiyer from heterodrugs.com (Mr.Pradeep Kumar Iyer Group Leader, Bio Tech, HRF) Date: Wed Oct 21 05:06:42 2009 Subject: [Glycosci] Querry regarding silver staining for glycoproteins Message-ID: <612b9b830910192126y4543a7bei502a574db6c2601d@mail.gmail.com> Dear All, I have this confusion on the silver staining of glycoproteins in IEF gels. I have observed that with increasing glycosylation, the intensity of the glycoproteins after silver stain decreases. I attribute this to the masking of the aminoacids by the glycan chain resulting in the decrease in efficiency of the silver stain. Has anyone done an IEF for deglycosylated molecule? Does it give a more intense band than the glycosylated one? My next experiment would be that but if that is observed by most of you, I would not waste my resources on doing that. Kindly give some inputs. Regards, Pradeep Iyer --=20 If you're not part of the solution, you're part of the precipitate =96 Henr= y J. Tillman From D.Lefeber from neuro.umcn.nl Mon Oct 26 06:40:25 2009 From: D.Lefeber from neuro.umcn.nl (D.Lefeber@neuro.umcn.nl) Date: Thu Oct 29 08:51:41 2009 Subject: [Glycosci] Querry regarding silver staining for glycoproteins In-Reply-To: <612b9b830910192126y4543a7bei502a574db6c2601d@mail.gmail.com> References: <612b9b830910192126y4543a7bei502a574db6c2601d@mail.gmail.com> Message-ID: <8C092D3F0CAD0B43BA8841B53C731A5A019CA880@UMCEXBE02.umcn.nl> Dear all, Does someone know an interested researcher for a post-doc on glycan mass spectrometry, as atteched? Kind regards, Dirk Please note address change! ----------------------------------------------------------------- Dr. D.J. Lefeber, PhD Clinical Biochemical Geneticist Glycosylation & Lysosomal disorders Department of Laboratory Medicine 830 - Laboratory of Genetic Endocrine and Metabolic Diseases Radboud University Nijmegen Medical Centre Geert Grooteplein 10 6525 GA Nijmegen The Netherlands D.Lefeber@neuro.umcn.nl phone: +31 24 3614428 / 3953 fax: +31 24 3618900 http://www.umcn.nl/lkn -----Oorspronkelijk bericht----- Van: glycosci-bounces@oat.bio.indiana.edu [mailto:glycosci-bounces@oat.bio.indiana.edu] Namens Mr.Pradeep Kumar Iyer Group Leader, Bio Tech, HRF Verzonden: dinsdag 20 oktober 2009 6:26 Aan: glycosci@magpie.bio.indiana.edu; methods@magpie.bio.indiana.edu Onderwerp: [Glycosci] Querry regarding silver staining for glycoproteins Dear All, I have this confusion on the silver staining of glycoproteins in IEF gels. I have observed that with increasing glycosylation, the intensity of the glycoproteins after silver stain decreases. I attribute this to the masking of the aminoacids by the glycan chain resulting in the decrease in efficiency of the silver stain. Has anyone done an IEF for deglycosylated molecule? Does it give a more intense band than the glycosylated one? My next experiment would be that but if that is observed by most of you, I would not waste my resources on doing that. Kindly give some inputs. Regards, Pradeep Iyer -- If you're not part of the solution, you're part of the precipitate - Henry J. Tillman _______________________________________________ Glycosci mailing list Glycosci@net.bio.net http://www.bio.net/biomail/listinfo/glycosci Het UMC St Radboud staat geregistreerd bij de Kamer van Koophandel in het handelsregister onder nummer 41055629. The Radboud University Nijmegen Medical Centre is listed in the Commercial Register of the Chamber of Commerce under file number 41055629. From pradeepiyer from heterodrugs.com Tue Oct 27 06:44:46 2009 From: pradeepiyer from heterodrugs.com (Mr.Pradeep Kumar Iyer Group Leader, Bio Tech, HRF) Date: Thu Oct 29 08:51:45 2009 Subject: [Glycosci] Sialic acid pH!!! Message-ID: <612b9b830910270444t40a8ce62u91ba6bd16d559199@mail.gmail.com> HEy there.. Can anyone tell the the change in pH (in terms of ph unit/siali= c acid molecule) of a glycoprotein on addition of a single sialic acid?!! Pradeep --=20 If you're not part of the solution, you're part of the precipitate =96 Henr= y J. Tillman From jparrish from ansci.wisc.edu Tue Oct 27 16:29:27 2009 From: jparrish from ansci.wisc.edu (joan parrish) Date: Thu Oct 29 08:51:48 2009 Subject: [Glycosci] Sequencers, synths, mass specs, MALDIs etc. available Message-ID: <2BADAB34-BF3F-4956-84B6-EA209668F45A@ansci.wisc.edu> Hello, We are trying to find a windows system to upgrade a Bruker Biflex III. Do you have any suggestions since Bruker no longer sells the system. Thanks for any info, Joan Joan Parrish Department of Animal Science Grants Facilitator jparrish@ansci.wisc.edu (608)263-7761