From bilder from berkeley.edu Mon Jan 5 20:08:46 2009 From: bilder from berkeley.edu (David Bilder) Date: Tue Jan 6 09:34:31 2009 Subject: [Drosophila] white singed bar;; ebony teaching stock? Message-ID: We have been using a white singed bar;; ebony stock for teaching undergraduate genetics but have recently lost it. If anyone has this stock, or a similar stock (multiply marked on X with recessives and dominants) I would love to hear about it. Many thanks, David Bilder (bilder@berkeley.edu) From brancojoana from hotmail.com Wed Jan 7 07:29:06 2009 From: brancojoana from hotmail.com (Joana Branco) Date: Wed Jan 7 12:15:01 2009 Subject: [Drosophila] Incubator/chamber Message-ID: Dear all, I will be setting up a new lab, and was thinking of installing a chamber (with controlled temperature and light/dark cycle), instead of using multiple incubators. Does anyone know any brand/vendor/type? Any suggestions on how to choose the best one? Thank you all. Best, Joana _________________________________________________________________ Windows LiveTM: Keep your life in sync. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t1_allup_explore_012009 From kuritaro from earthlink.net Thu Jan 8 22:07:43 2009 From: kuritaro from earthlink.net (Chris Jones) Date: Fri Jan 9 08:58:46 2009 Subject: [Drosophila] strong eye Gal4 line? Message-ID: A student and I are trying to examine UAS expression of a transgene in eyes and wings. For the eyes, we got line 9146 from Bloomington (w[1118]; P{GMR-GAL4.w[-]}2/CyO), which shows some expression using a UAS-GFP construct, but expression appears weak. What recommendations do folks have for a strong eye-specific driver line? Many thanks, Chris Jones cjones@moravian.edu From scottatg from aol.com Thu Jan 8 21:35:33 2009 From: scottatg from aol.com (scottatg@aol.com) Date: Fri Jan 9 08:59:27 2009 Subject: [Drosophila] Incubator/chamber (Joana Branco) In-Reply-To: <200901081704.n08H4Uj28427@net.bio.net> References: <200901081704.n08H4Uj28427@net.bio.net> Message-ID: <8CB401A1486749A-10DC-1082@MBLK-M16.sysops.aol.com> joana, percival scientific is by far the best. www.percivalscientific.com. If you are doing drosophila research you MUST get phenolic coated coils for the refrigeration unit. Incubator/chamber (Joana Branco) ?Ask me about Miele Glassware Washers. Best Regards, Scott Saveleski Atlantic Technology Group, Inc. 1906 North Hamilton Street, Suite G Richmond, Va. 23230 1-800-359-5153 x2 410-371-8740 cell -----Original Message----- From: dros-request@oat.bio.indiana.edu To: dros@magpie.bio.indiana.edu Sent: Thu, 8 Jan 2009 12:04 pm Subject: Dros Digest, Vol 45, Issue 2 Send Dros mailing list submissions to dros@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/dros or, via email, send a message with subject or body 'help' to dros-request@net.bio.net You can reach the person managing the list at dros-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Dros digest..." Today's Topics: 1. Incubator/chamber (Joana Branco) ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 Jan 2009 12:29:06 +0000 From: Joana Branco Subject: [Drosophila] Incubator/chamber To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear all, I will be setting up a new lab, and was thinking of installing a chamber (with controlled temperature and light/dark cycle), instead of using multiple incubators. Does anyone know any brand/vendor/type? Any suggestions on how to choose the best one? Thank you all. Best, Joana _________________________________________________________________ Windows LiveTM: Keep your life in sync. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t1_allup_explore_012009 ------------------------------ _______________________________________________ Dros mailing list Dros@net.bio.net http://www.bio.net/biomail/listinfo/dros End of Dros Digest, Vol 45, Issue 2 *********************************** From nsatkinson from gmail.com Fri Jan 9 10:51:35 2009 From: nsatkinson from gmail.com (Nigel Atkinson) Date: Fri Jan 9 11:42:44 2009 Subject: [Drosophila] Incubator/chamber (Joana Branco) In-Reply-To: <8CB401A1486749A-10DC-1082@MBLK-M16.sysops.aol.com> References: <200901081704.n08H4Uj28427@net.bio.net> <8CB401A1486749A-10DC-1082@MBLK-M16.sysops.aol.com> Message-ID: I would also get the humidity control. I was advised by Percival to skip it and now I am looking at retrofitting two incubators. Nigel On Jan 8, 2009, at 8:35 PM, scottatg@aol.com wrote: > joana, > > percival scientific is by far the best. www.percivalscientific.com. > If you are doing drosophila research you MUST get phenolic coated > coils for the refrigeration unit. > > > Incubator/chamber (Joana Branco) > > > > > ?Ask me about Miele Glassware Washers. > > Best Regards, > > Scott Saveleski > Atlantic Technology Group, Inc. > 1906 North Hamilton Street, Suite G > Richmond, Va. 23230 > 1-800-359-5153 x2 > 410-371-8740 cell > > > -----Original Message----- > From: dros-request@oat.bio.indiana.edu > To: dros@magpie.bio.indiana.edu > Sent: Thu, 8 Jan 2009 12:04 pm > Subject: Dros Digest, Vol 45, Issue 2 > > > > Send Dros mailing list submissions to > dros@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/dros > or, via email, send a message with subject or body 'help' to > dros-request@net.bio.net > > You can reach the person managing the list at > dros-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Dros digest..." > > > Today's Topics: > > 1. Incubator/chamber (Joana Branco) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 7 Jan 2009 12:29:06 +0000 > From: Joana Branco > Subject: [Drosophila] Incubator/chamber > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > > > Dear all, > > I will be setting up a new lab, and was thinking of installing a > chamber (with > controlled temperature and light/dark cycle), instead of using > multiple > incubators. > > Does anyone know any brand/vendor/type? > > Any suggestions on how to choose the best one? > > Thank you all. > > Best, > > Joana > > > > > > > _________________________________________________________________ > Windows LiveTM: Keep your life in sync. > http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t1_allup_explore_012009 > > ------------------------------ > > _______________________________________________ > Dros mailing list > Dros@net.bio.net > http://www.bio.net/biomail/listinfo/dros > > End of Dros Digest, Vol 45, Issue 2 > *********************************** > > _______________________________________________ > Dros mailing list > Dros@net.bio.net > http://www.bio.net/biomail/listinfo/dros From biospace from noster-it.com Sat Jan 17 10:28:41 2009 From: biospace from noster-it.com (biospace) Date: Sat Jan 17 11:36:38 2009 Subject: [Drosophila] Flies May Reveal Evolutionary Step To Live Birth Message-ID: <89ecefd7-60af-4f7f-ba70-e0967b48292b@r36g2000prf.googlegroups.com> A species of fruit fly from the Seychelles Islands often lays larvae instead of eggs, UC San Diego biologists have discovered. Clues to how animals switch from laying eggs to live birth may be found in the well- studied species’ ecology and genes. The fly is one of a dozen species of Drosophila to have recently had their genomes sequenced, information that should provide abundant opportunities for identifying genetic changes that cause females of this species, and not others, to retain their fertilized eggs until they are ready to hatch. The result was so surprising that the scientists initially thought it was a mistake. “The student who was timing things came a said ‘wow, these eggs in this species really develop quickly,’ sometimes in less than an hour. That’s not possible,” said Therese Markow, a professor of biology who led the project. “When I went and actually looked at them I saw that they were depositing something that was very advanced, that hatched into a larva right away. In several cases they were hatching as they were being laid.” Even those Seychelles fly eggs that emerged unhatched were at an advanced state of development, the team reports in forthcoming issue of the Journal of Evolutionary Biology. Most larvae emerged within two hours compared to an average of nearly 23 hours for the other 10 species in the study. Tonny -------------- More bio-med news & videos Portal to share biological information-data between people http://biospace.ethz.ch From dunipace from caltech.edu Wed Jan 21 17:29:14 2009 From: dunipace from caltech.edu (Leslie Dunipace) Date: Thu Jan 22 08:55:35 2009 Subject: [Drosophila] recombineering Message-ID: <244B9F18AC6E4D85B439EE748FF64092@DunipacePC> Ed: I have been using the galK system for a few months now and would be happy to talk to you if you are still having problems. For the most part the system has been very robust in my hands. The only times I have had problems are when I made a mistake in designing the inserts or one time when I had contamination. What sort of problems are you having? Leslie From hbellen from bcm.tmc.edu Sun Jan 25 17:08:45 2009 From: hbellen from bcm.tmc.edu (Bellen, Hugo J) Date: Mon Jan 26 10:00:17 2009 Subject: [Drosophila] Searching for Ashburner's Drosophila: A Laboratory Handbook Message-ID: <71A777D260335247BB669E57EDF67F4229CA44@BCMEVS16.ad.bcm.edu> Hugo J. Bellen, DVM PhD Howard Hughes Medical Institute Program in Developmental Biology Baylor College of Medicine One Baylor Plaza, Rm. T628 Mailstop BCM235 Houston, TX 77030 Tel: 713-798-5272 Fax: 713-798-3694 email: hbellen@bcm.tmc.edu http://flypush.imgen.bcm.tmc.edu/ From t.buechling from dkfz-heidelberg.de Thu Jan 29 06:05:33 2009 From: t.buechling from dkfz-heidelberg.de (Tina Buechling) Date: Thu Jan 29 09:27:53 2009 Subject: [Drosophila] help with phenotype Message-ID: <1493B38F-1BF5-4767-B742-47E41A4FEBF1@dkfz.de> Hi guys, I was wondering whether anybody of you knows which genes/pathways are involved in the formation of the scutellar bristles? I have a phenotype, where only the four bristles as in the sco marker are missing and was wondering what might cause that. Thanks a lot in advance. Best, Tina ----------------------------------------------------- Tina Buechling German Cancer Research Center Signaling and Functional Genomics (B110) Im Neuenheimer Feld 580 69120 Heidelberg/Germany +49 6221 421955 t.buechling@dkfz.de From SPowell from apebridgepath.com Fri Jan 30 16:46:27 2009 From: SPowell from apebridgepath.com (Shane Powell) Date: Sat Jan 31 10:32:54 2009 Subject: [Drosophila] Sorvall Centirfuge schematics (Adam Hall) Message-ID: Hi Adam, I work for a company called Bridgepath scientific and I was wonder= ing if you ever found a service manual or schematics for your RC. I am havi= ng problems with mine and that would be a great help, if you did find them = please let me know how I can get a copy. Thanks a lot Shane Powell No virus found in this outgoing message. Checked by AVG. Version: 7.5.552 / Virus Database: 270.10.16/1925 - Release Date: 1/30/2009= 7:37 AM