From rj2152 from gmail.com Sun Nov 2 17:06:12 2008 From: rj2152 from gmail.com (Rohit Joshi) Date: Mon Nov 3 08:41:44 2008 Subject: [Drosophila] FRT-Ecdysone receptor Message-ID: hi! fly people I was wondering if any one is aware of existence of a FRT-Ecdysone receptor line. EcR is on 42A9 but lately I saw FRT40A which made me think if FRT40A-EcR exist. Idea is to be a able to do some clonal analysis with EcR mutants using MARCM, thanks rohit From Maki.Asano from osumc.edu Sat Nov 15 16:15:57 2008 From: Maki.Asano from osumc.edu (Asano, Maki) Date: Mon Nov 17 06:43:57 2008 Subject: [Drosophila] (no subject) Message-ID: <6C0201147C11F14AB5A5157C7BDABF600101CA31@msxc06.OSUMC.EDU> Dear All: I'm setting up new lab at The Comprehensive Cancer Center, Ohio State University. Currently I have two postdocs working with me. I have several positions availble for either Postdocs or Research Associate/Assistant (Technicians), as well as for graduate students. Previous experience in Drosophila research is not necessary. Those interested shall apply directly. We work on Cell Cycle regulation during Drosophila development. Our main focus is on the control mechanisms of DNA replication initiation. We are also starting to look into multi-function of ORC1 and Cdc6 outside of DNA replication. Recent Publications: Park SY, and Asano M. The origin recognition complex is dispensable for endoreplication in Drosophila. Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12343-8. Epub 2008 Aug 18. Narbonne-Reveau K, Senger S, Pal M, Herr A, Richardson HE, Asano M, Deak P, and Lilly MA. APC/CFzr/Cdh1 promotes cell cycle progression during the Drosophila endocycle. Development. 2008 Apr;135(8):1451-61. Epub 2008 Mar 5. Wu Q, Guo Y, Yamada A, Perry JA, Wang MZ, Araki M, Freel CD, Tung JJ, Tang W, Margolis SS, Jackson PK, Yamano H, Asano M, and Kornbluth S. A role for Cdc2- and PP2A-mediated regulation of Emi2 in the maintenance of CSF arrest. Curr Biol. 2007 Feb 6;17(3):213-24. Okudaira K, Ohno K, Yoshida H, Asano M, Hirose F, and Yamaguchi M. Transcriptional regulation of the Drosophila orc2 gene by the DREF pathway. Biochim Biophys Acta. 2005 Dec 30;1732(1-3):23-30. Araki M, Yu H, and Asano M. A novel motif governs APC-dependent degradation of Drosophila ORC1 in vivo. Genes Dev. 2005 Oct 15;19(20):2458-65. Epub 2005 Sep 29. Araki M, Wharton RP, Tang Z, Yu H, and Asano M. Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila. EMBO J. 2003 Nov 17;22(22):6115-26. Best regards, Maki Maki Asano, M.D., Ph.D. Assistant Professor Tumor Microenvironment Program OSU Cancer Center Department of Molecular Cellular Biochemistry College of Medicine, Ohio State University 812 Biomedical Research Tower (BRT) 460 W. 12th Avenue, Columbus OH 43210 614-688-4164 Fax : 614-688-4181 maki.asano@osumc.edu From jgreen from cicbiogune.es Mon Nov 17 11:00:15 2008 From: jgreen from cicbiogune.es (Jon Green) Date: Tue Nov 18 08:44:39 2008 Subject: [Drosophila] Drosophila larvae injections Message-ID: Dear all, I am interested in doing some transplant experiments on drosophila 3rd instar larvae. Is there anyone still actively using this technique or have used it in the past who could give me a few pointers? Thanks and regards, Jon This e-mail is from CIC bioGUNE. The e-mail and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any unauthorised dissemination or copying of this e-mail or its attachments, and any use or disclosure of any information contained in them, is strictly prohibited and may be illegal. If you have received this e-mail in error, please notify or telephone + 34 944 06 13 00 and delete it from your system. From j.a.t.dow from bio.gla.ac.uk Wed Nov 19 08:37:12 2008 From: j.a.t.dow from bio.gla.ac.uk (Julian Dow) Date: Wed Nov 19 09:25:56 2008 Subject: [Drosophila] FlyAtlas.org: 5 new tissues added Message-ID: <49241688.6050608@bio.gla.ac.uk> http://flyatlas.org is an Affymetrix microarray-based atlas of gene expression in multiple tissues of adult and larval Drosophila melanogaster. It been accessed by over 5000 users in the last three months, from nearly everywhere except Alaska. We are delighted to announce the addition of multiple new tissues: - larval salivary gland - larval midgut - larval hindgut (all from feeding third instar larvae) - adult spermatheca from 7-day old mated females - adult spermatheca from 7-day old virgin females Data quality We take great pride in the purity of the dissections, as evidenced by high enrichments relative to whole fly. Here are some interesting selections: Larval salivary gland Most enriched (16217x !): anonymous gene CG12715 Highly enriched for: puparial adhesion and sgs genes Most enriched transcription factor: sage (at 240x) Most enriched receptor: ETH receptor (at 51x). I'd always wondered where it was! Larval midgut Most enriched (10755x !): anonymous gene CG5084 Enriched for: structural components of peritrophic membrane Most enriched TF: larval-specific transcript of br (33x) Most enriched receptor: methuselah-like 9 (13x) Larval hindgut Most enriched (at just 6331x): lcp4 Enriched in: larval cuticle proteins (Lcps) and larval serum proteins (lsps) Most enriched TF: orthopedia (56x), previously implicated in CNS development. Hence the phrase "His brains are in his ...." Most enriched receptor: leucokinin receptor (69x) Virgin spermatheca Most enriched: anonymous serine-type endopeptidase CG17234 (334x) Enriched in: serine-type endopeptidases Most enriched TF: pannier (38x) Most enriched receptor: leucine-rich repeat-containing G protein-coupled receptor 2 (rk) (10x) Mated spermatheca Most enriched: anonymous serine-type endopeptidase CG17234 (650x) Enriched in: serine-type endopeptidases Most enriched TF: pannier (45x) Most enriched receptor: leucine-rich repeat-containing G protein-coupled receptor 2 (rk) (14x) You may be wondering whether it was worth burning the extra $2000 for separate mated and virgin spermatheca. But this provides a nice internal control for consistency, and differences between the two are very informative. For scriptwriters, programmatic calls to the database are possible, e.g. http://flyatlas.org/atlas.cgi?name=CG1147 You can search with FBgn, CG number, Affy oligo, gene symbol or free text. These data were prepared and placed in the public domain as a service to the Drosophila community, by the UK’s Biotechnology and Biological Sciences Research Council (BBSRC) through their Investigating Gene Function (IGF) and bioinformatics and biological resources (BBR) initiatives. Over the last 4 years, our facility has provided 1000 free Affymetrix arrays to the UK fly community. Further details at: http://www.mblab.gla.ac.uk/igf/ Enjoy! Julian Dow (j.a.t.dow@bio.gla.ac.uk) -------------- next part -------------- No virus found in this incoming message. Checked by AVG. Version: 8.0.100 / Virus Database: 270.4.1/1515 - Release Date: 23/06/2008 19:16 From Alexei.Tulin from fccc.edu Thu Nov 20 13:22:24 2008 From: Alexei.Tulin from fccc.edu (Tulin, Alexei) Date: Thu Nov 20 14:56:12 2008 Subject: [Drosophila] drosophila research positions are available Message-ID: <521A7FAFA50F3B4DA23C51FA10576D90EA3C16@rex1.ritf.fccc.edu> Technician and postdoctoral research positions are available in the Epigenetics and Progenitor Cells Program at Fox Chase Cancer Center (Philadelphia, PA, USA). Researchers will study the molecular mechanisms of the chromatin remodeling and transcriptional activation in Drosophila melanogaster. The applicant may be at MSc or PhD level and should have experience in Drosophila genetics research. Experience in confocal microscopy, live-cell imaging (FRAP, FRET) and genomics/proteomics would be helpful. Positions available immediately!!! The choice of the project will be discussed with a candidate during the interview. Please send current CV and 3 letters of reference to: Alexei Tulin, PhD Epigenetics and Progenitor Cells Program Fox Chase Cancer Center 333 Cottman Ave. R362, Philadelphia, PA 19111, e-mail: Alexei.Tulin@fccc.edu From Stephanie_Mohr from hms.harvard.edu Tue Nov 25 11:22:35 2008 From: Stephanie_Mohr from hms.harvard.edu (Stephanie Mohr) Date: Tue Nov 25 13:58:07 2008 Subject: [Drosophila] Transgenic RNAi Project (TRiP) Accepting Gene Nominations Message-ID: <55947579-A237-498B-BE50-E46C00DB6C6B@hms.harvard.edu> Is your favorite fly gene an unknown? Not represented by an allele at Bloomington? Mutations lead to pleiotropic defects that prevent study in your tissue of interest? Would otherwise benefit from a robust, inducible transgenic RNAi hairpin approach? The NIH-funded Transgenic RNAi Project (TRiP) at Harvard Medical School is accepting community nominations. Based on these gene nominations and other criteria (including input from Bloomington), the TRiP will decide for what genes they will generate hairpin constructs and corresponding transgenic flies. To learn more about the TRiP and to make nominations, please visit the "Nominations" section of the TRiP webpages (within the Drosophila RNAi Screening Center's website) at http://flyrnai.org/TRiP-HOME.html To learn about exsiting TRiP stocks, please visit the "TRiP Stocks" section of the webpages at http://flyrnai.org/TRiP-HOME.html To learn about screening the existing collection at HMS, please visit the "Screening Center" section at http://flyrnai.org/TRiP-HOME.html To learn more about ongoing and new services of the Drosophila RNAi Screening Center, visit http://flyrnai.org/ Thank you, The TRiP Team (N. Perrimon, PI; S. Mohr, DRSC Director; L. Perkins, TRiP Chief; H. Shim, TRiP Manager) From info from noster-it.com Sun Nov 30 03:48:19 2008 From: info from noster-it.com (Dokorek) Date: Sun Nov 30 09:22:17 2008 Subject: [Drosophila] Human Obesity Genes Revealed Based On Fly Experiments Message-ID: <3803af2c-4f47-4f93-949d-d966552f56dc@j39g2000yqn.googlegroups.com> A study of 228 women has revealed genetic variants responsible for body shape. Based on work in the fruit fly Drosophila melanogaster, research published in the open access journal BMC Genetics identifies natural variation in the human LAMA5 gene as a key determinant of weight. As the prevalence of obesity and related health problems continues to increase worldwide, there is considerable effort being devoted to identify genetic mechanisms that control fat storage. Maria De Luca led a team from the University of Alabama at Birmingham, USA, who identified candidate genes using different strains of Drosophila. On the basis of the results of these fly experiments, the research team then tested three common variations in the human LAMA5 gene and discovered two gene variants that were associated with body shape, one in women of European American descent and the other affecting women of American African descent. Dokorek -------------- Portal to share biological information-data between people http://biospace.ethz.ch