Hi,
does anybody know a good setting on the Bio-Rad GenePulser Xcell
apparatus to electroporate Chlamy?
We were used to electroporate the algae with the Eppendorf Multiporator
in prokaryotic mode at 750V and 5ms but our numerous attempts to
reproduce that on the new apparatus failed.
We are using the sucrose/starch protocol established to electroporate a
cw15 strain.
Thank you
David
--
David Dauvillée
*"Biochemical Genetics and Functional Biology"*
Unité de Glycobiologie Structurale et Fonctionnelle
UMR 8576 CNRS
USTL / Bât C9
59650 Villeneuve d'Ascq
FRANCE
Tel: +33 3 20 43 65 43
Fax: +33 3 20 43 65 55
David.Dauvillee from univ-lille1.fr
