The solution is to use glucose instead of sucrose in the
transformation media. You can see a more detailed protocol in our
webpage http://www4.ncsu.edu/~jmalonso/Alonso_Lab/Protocols.html
I hope this helps.
Jose
Jose Alonso
Phone: (919)515 5729
Fax: (919) 515 3355
Email: jmalonso from unity.ncsu.edu
Web: http://www4.ncsu.edu/~jmalonso
On Mar 20, 2009, at 12:02 PM, Hernandez Verdeja, Tamara wrote:
> Hi everybody,
>>>> I am trying to transform Arabidopsis with a JAtY clone, from the
> John Innes Center Genome Lab and I am not getting any transformant.
> I am using the standard floral-dip protocol with 5% sucrose and the
> Agrobacterium strain C58C1. Could anyone give me some advice? Some
> specific transformation media, Agro strain...
>>>> Thank you very much in advance,
>>>> Rafael Catala
>>>>>> Rafael Catala
>>catala from cib.csic.es>> Centro de Investigaciones Biológicas (CIB-CSIC)
> Centre for Biological Research---Spanish Research Council
> Ramiro de Maeztu, 9
> Campus Universidad Complutense
> 28040 Madrid
> SPAIN
>>> _______________________________________________
> Arab-gen mailing list
>Arab-gen from net.bio.net>http://www.bio.net/biomail/listinfo/arab-gen
