On Jun 15, 7:40 am, "Abo-Ogiala, Atef" <Atef.Abo-Ogi... from forst.uni-
goettingen.de> wrote:
> Please I need answer for the same question:
>> I want to find the orientation of T-DNA insertion in some mutants of
> Arabidopsis.
>> Best regards
>> Atef Abo-Ogiala
>> PhD student
>> Büsgen-Institut
>> Forstbotanik und Baumphysiologie
>> Georg-August Universität
>> Büsgenweg 2
>> 37077 Goettingen
>> Germany
>> Tel: +49 (0)551 - 39 9362
>> Fax: +49 (0)551 - 39 22705
Depending from which mutant bank you request the mutants is possible
to see the orientation of the insertion on the SIGnAL T-DNA Express
tool (http://signal.salk.edu/cgi-bin/tdnaexpress), just need the AGI
number of the interrupted gene and the code of the mutant. The page
will display the gene orientation on the genome and the orientation of
all T-DNA insertion mapped to that gene. If you have your own mutant
bank generated by T-DNA insertion, to find the T-DNA orientation you
should perform a TAIL-PCR using the T-DNA that you prefer.
I hope this help...
