Mr, Abo-Ogiala
You were targeting seeds for segregated lines. Do you have any PCR
pictures we could see? How did you genotype these lines?
A homozygous line has being already isolated for gene At5g02020, you
can request SALK_064028C to ABRC.
Thanks
Cesar Barragan
The Salk Institute for Biological Studies
Genomic Analysis Laboratory
10010 North Torrey Pines Road
La Jolla, CA 92037
Tel 858-453-4100 ext 1108
barragan from salk.edu
On Jul 15, 2009, at 11:11 AM, Ronan OMalley wrote:
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> From: <arab-gen-request from oat.bio.indiana.edu>
> Date: Wed, Jul 15, 2009 at 9:03 AM
> Subject: Arab-gen Digest, Vol 51, Issue 7
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>>> Today's Topics:
>> 1. At5g02020 (Abo-Ogiala, Atef)
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>> Message: 1
> Date: Tue, 14 Jul 2009 11:14:26 +0200
> From: "Abo-Ogiala, Atef" <Atef.Abo-Ogiala from forst.uni-goettingen.de>
> Subject: [Arabidopsis] At5g02020
> To: <arab-gen from magpie.bio.indiana.edu>
> Message-ID:
> <99392551BB49ED458ACFE796416D3189E1DBD3 from VS3.exc.top.gwdg.de>
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>> Hello,
>> We ordered SALK_040413 of the gene At5g02020. We have problem with
> this line.
> The whole plants that we get from the seeds are wild type. Please
> if you have
> homozygous line for At5g02020 gene except Salk_146631 tell me from
> which
> source I can get it.
>> Best regards
>> Atef Abo-Ogiala
>> PhD student
>> Büsgen-Institut
>> Forstbotanik und Baumphysiologie
>> Georg-August Universität
>> Büsgenweg 2
>> 37077 Goettingen
>> Germany
>>>> Tel: +49 (0)551 - 39 9362
>> Fax: +49 (0)551 - 39 22705
>>>>>> ------------------------------
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