From <@VM.NRC.CA:Nash@biologysx.lan.nrc.ca> Mon Feb 24 14:25:21 1992 Received: from IUBIO.BIO.INDIANA.EDU by sunflower.bio.indiana.edu (4.1/9.5jsm) id AA21554; Mon, 24 Feb 92 14:25:19 EST From: Subject: Numclone utility (IBM-PC and clones). To: Date: Mon, 24 Feb 92 11:48:00 EST Message-Id: <29A94435@coursmtp.nrc.ca> Encoding: 35 TEXT, 477 TEXT X-Mailer: Microsoft Mail V3.0 (beta-1) X-Ms-Attachment: NCLONE1.UUE 30223 02-24-1992 11:36 Status: R G'day netfolk... It sure is quiet around here. To lighten up a dull Northern winter, I'll pass along something I wrote for fun... it's more of a utility than a "real" program. NUMCLONE estimates the number of clones one has to screen from a genomic library in order to find a desired clone. I only wrote it because I keep on losing my copy of the formula... I KNOW where my computer is!! You need to supply: - desired probability, e.g .99, or .90, etc., - genome size (in megabases), - insert size (in kilobases). The program will work on IBM PC, XT, and AT clones using CGA, EGA, VGA, MDA or the ubiquitous HGC. The algorithm was taken from page 9-3 of Molecular Cloning: A Laboratory Manual (second edition) by Sambrook, Fritsch and Maniatis, Cold Spring Harbor Press, Cold Spring Harbor, 1989. The UUEncoded program UUDecodes to a self-extracting archive file (generated by LHA v2.13), which contains NUMCLONE.EXE and NUMCLONE.DOC. cheers, John.