>From LISS@alf1.ngate.uni-regensburg.de Wed Jun 23 10:40:10 1993 To: archive@bio.indiana.edu >From: "Michael Liss" Subject: i had problems via ftp, try this way now Priority: normal X-Mailer: Pegasus Mail v2.3 (R5). Content-Length: 1987 X-Lines: 49 Status: RO INTRON ANALYZER Version 3.0 Written by Michael Liss University of Regensburg Germany (c) 1992 Inspired by an article of Witold Filipowicz (Friedrich Miescher-Institut, CH-4002 Basel, Switzerland), which documents that there are basic differences in the base composition of introns from animals and plants, we wanted to examine the introns of Volvox carteri, a multi-cellular alga, to find regularities in them. And this is exactly what this program does. From a given list of at most 350 introns you can explore them by ligning them up either at the 5' or at the 3'end or you may study the adjoining exon-parts. The program will then build a consensus-sequence which shows what base or type of base (Pur/Pyr, AT/GC) is most frequent in each position. Also a graphic plot is possible, where these frequencies are displayed in percent. System requirements INTANA needs an IBM compatible machine with MS- or PC-DOS 3.0 or later. The connected monitor can be color or b/w. An EGA card is suggested to view graphics. The standard configuration supports an Epson compatible matrix printer, although any other printer can be controled by changing the INTANA.CFG file. Files after unziping INTANA.ZIP: - INTANA.EXE : The INTron ANAlyzing program - INTANA.CFG : The configuration file used by INTANA.EXE - INTANA.DOC : Documentation - INTCUT.EXE : The INTron CUTting program used to extract the introns from given sequence files Sample files to test INTANA: - NRED.SEQ : Sequence of Nitrate-reductase from Volvox carteri - UBI.SEQ : Sequence of Ubiquitin from Volvox carteri - VOLVOX.CUT : Index file used by INTCUT.EXE (containing "NRED.SEQ" & "UBI.SEQ")