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2.12. BLOSUM62 amino acid symbol comparison table


Dayhoff table (Schwartz, R. M. and Dayhoff, M. O. [1979] in Atlas of Protein Sequence and Structure, Dayhoff, M. O. Ed, pp. 353-358, National Biomedical Research Foundation, Washington D.C.) rescaled by dividing each value by the sum of its row and column, and normalizing to a mean of 0 and standard deviation of 1.0. The value for FY (Phe-Tyr) = RW =1.425. Perfect matches are set to 1.5 and no matches on any row are better than perfect matches.

NB: This table was derived from an analysis of globular proteins!

A B C D E F G H I K L M N P Q R S T V W Y Z .

1.5 0.2 0.3 0.3 0.3 -0.5 0.7 -0.1 0.0 0.0 -0.1 0.0 0.2 0.5 0.2 -0.3 0.4 0.4 0.2 -0.8 -0.3 0.2 A

1.1 -0.4 1.1 0.7 -0.7 0.6 0.4 -0.2 0.4 -0.5 -0.3 1.1 0.1 0.5 0.1 0.3 0.2 -0.2 -0.7 -0.3 0.6 B

1.5 -0.5 -0.6 -0.1 0.2 -0.1 0.2 -0.6 -0.8 -0.6 -0.3 0.1 -0.6 -0.3 0.7 0.2 0.2 -1.2 1.0 -0.6 C

1.5 1.0 -1.0 0.7 0.4 -0.2 0.3 -0.5 -0.4 0.7 0.1 0.7 0.0 0.2 0.2 -0.2 -1.1 -0.5 0.9 D

1.5 -0.7 0.5 0.4 -0.2 0.3 -0.3 -0.2 0.5 0.1 0.7 0.0 0.2 0.2 -0.2 -1.1 -0.5 1.1 E

1.5 -0.6 -0.1 0.7 -0.7 1.2 0.5 -0.5 -0.7 -0.8 -0.5 -0.3 -0.3 0.2 1.3 1.4 -0.7 F

1.5 -0.2 -0.3 -0.1 -0.5 -0.3 0.4 0.3 0.2 -0.3 0.6 0.4 0.2 -1.0 -0.7 0.3 G

1.5 -0.3 0.1 -0.2 -0.3 0.5 0.2 0.7 0.5 -0.2 -0.1 -0.3 -0.1 0.3 0.5 H

1.5 -0.2 0.8 0.6 -0.3 -0.2 -0.3 -0.3 -0.1 0.2 1.1 -0.5 0.1 -0.2 I

1.5 -0.3 0.2 0.4 0.1 0.4 0.8 0.2 0.2 -0.2 0.1 -0.6 0.4 K

1.5 1.3 -0.4 -0.3 -0.1 -0.4 -0.4 -0.1 0.8 0.5 0.3 -0.2 L

1.5 -0.3 -0.2 0.0 0.2 -0.3 0.0 0.6 -0.3 -0.1 -0.1 M

1.5 0.0 0.4 0.1 0.3 0.2 -0.3 -0.3 -0.1 0.4 N

1.5 0.3 0.3 0.4 0.3 0.1 -0.8 -0.8 0.2 P

1.5 0.4 -0.1 -0.1 -0.2 -0.5 -0.6 1.1 Q

1.5 0.1 -0.1 -0.3 1.4 -0.6 0.2 R

1.5 0.3 -0.1 0.3 -0.4 0.0 S

1.5 0.2 -0.6 -0.3 0.1 T

1.5 -0.8 -0.1 -0.2 V

1.5 1.1 -0.8 W

1.5 -0.6 Y

1.1 Z

NB: This default table will probably be replaced by the BLOSUM62 table in later releases of the GCG package. ($ FETCH BLOSUM62.CMP)

2.12.1. Analysis of symbol comparison values of PAM250

Reference: Henikoff, S. and Henikoff, J. G. (1992). Amino acid substitution matrices from protein blocks. Proc. Natl. Acad. Sci. USA 89: 10915-10919.

A B C D E F G H I K L M N P Q R S T V W X Y Z ..

4.0 -2.0 0.0 -2.0 -1.0 -2.0 0.0 -2.0 -1.0 -1.0 -1.0 -1.0 -2.0 -1.0 -1.0 -1.0 1.0 0.0 0.0 -3.0 -1.0 -2.0 -1.0 A

6.0 -3.0 6.0 2.0 -3.0 -1.0 -1.0 -3.0 -1.0 -4.0 -3.0 1.0 -1.0 0.0 -2.0 0.0 -1.0 -3.0 -4.0 -1.0 -3.0 2.0 B

9.0 -3.0 -4.0 -2.0 -3.0 -3.0 -1.0 -3.0 -1.0 -1.0 -3.0 -3.0 -3.0 -3.0 -1.0 -1.0 -1.0 -2.0 -1.0 -2.0 -4.0 C

6.0 2.0 -3.0 -1.0 -1.0 -3.0 -1.0 -4.0 -3.0 1.0 -1.0 0.0 -2.0 0.0 -1.0 -3.0 -4.0 -1.0 -3.0 2.0 D

5.0 -3.0 -2.0 0.0 -3.0 1.0 -3.0 -2.0 0.0 -1.0 2.0 0.0 0.0 -1.0 -2.0 -3.0 -1.0 -2.0 5.0 E

6.0 -3.0 -1.0 0.0 -3.0 0.0 0.0 -3.0 -4.0 -3.0 -3.0 -2.0 -2.0 -1.0 1.0 -1.0 3.0 -3.0 F

6.0 -2.0 -4.0 -2.0 -4.0 -3.0 0.0 -2.0 -2.0 -2.0 0.0 -2.0 -3.0 -2.0 -1.0 -3.0 -2.0 G

8.0 -3.0 -1.0 -3.0 -2.0 1.0 -2.0 0.0 0.0 -1.0 -2.0 -3.0 -2.0 -1.0 2.0 0.0 H

4.0 -3.0 2.0 1.0 -3.0 -3.0 -3.0 -3.0 -2.0 -1.0 3.0 -3.0 -1.0 -1.0 -3.0 I

5.0 -2.0 -1.0 0.0 -1.0 1.0 2.0 0.0 -1.0 -2.0 -3.0 -1.0 -2.0 1.0 K

4.0 2.0 -3.0 -3.0 -2.0 -2.0 -2.0 -1.0 1.0 -2.0 -1.0 -1.0 -3.0 L

5.0 -2.0 -2.0 0.0 -1.0 -1.0 -1.0 1.0 -1.0 -1.0 -1.0 -2.0 M

6.0 -2.0 0.0 0.0 1.0 0.0 -3.0 -4.0 -1.0 -2.0 0.0 N

7.0 -1.0 -2.0 -1.0 -1.0 -2.0 -4.0 -1.0 -3.0 -1.0 P

5.0 1.0 0.0 -1.0 -2.0 -2.0 -1.0 -1.0 2.0 Q

5.0 -1.0 -1.0 -3.0 -3.0 -1.0 -2.0 0.0 R

4.0 1.0 -2.0 -3.0 -1.0 -2.0 0.0 S

5.0 0.0 -2.0 -1.0 -2.0 -1.0 T

4.0 -3.0 -1.0 -1.0 -2.0 V

11.0 -1.0 2.0 -3.0 W

-1.0 -1.0 -1.0 X

7.0 -2.0 Y

5.0 Z

To use this table in place of the default for Bestfit or Gap (but beware of the default gap penalties, they may be too low):

$ Bestfit/Data1=genmoredata:blosum62.cmp


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