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2.11. PAM250 amino acid symbol comparison table

Default symbol comparison table used by BESTFIT for the comparison of nucleic acid sequences. BESTFIT uses the method of Smith/Waterman to make alignments.X's and N's are treated as matches to any IUB ambiguity symbol (score = 1.0). All mismatches for IUB symbols are -0.9, making BESTFIT clip off the best fitting segment at the point where the sequences stop being very similar.

A B C D G H K M N R S T U V W X Y ..

1.0 -0.9 -0.9 1.0 -0.9 1.0 -0.9 1.0 1.0 1.0 -0.9 -0.9 -0.9 1.0 1.0 1.0 -0.9 A

1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 B

1.0 -0.9 -0.9 1.0 -0.9 1.0 1.0 -0.9 1.0 -0.9 -0.9 1.0 -0.9 1.0 1.0 C

1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 D

1.0 -0.9 1.0 -0.9 1.0 1.0 1.0 -0.9 -0.9 1.0 -0.9 1.0 -0.9 G

1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 H

1.0 -0.9 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 K

1.0 1.0 1.0 1.0 -0.9 -0.9 1.0 1.0 1.0 1.0 M

1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 N

1.0 1.0 -0.9 -0.9 1.0 1.0 1.0 -0.9 R

1.0 -0.9 -0.9 1.0 -0.9 1.0 1.0 S

1.0 1.0 -0.9 1.0 1.0 1.0 T

1.0 -0.9 1.0 1.0 1.0 U

1.0 1.0 1.0 1.0 V

1.0 1.0 1.0 W

1.0 1.0 X

1.0 Y


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