MAPSORT/fragment creates a file that allows PLASMIDMAP to display boxes for the sequence range between cut sites. This is called "block" display as opposed to the "tick" default display.
(PLASMIDMAP /remarks=0 removes the text below the circle, /nocaption removes the caption to the left.)
$ Mapsort/fragment/Infile=platelet.seq
(Choose just two or three enzymes)
$ Plasmidmap/remarks=0/nocaption platelet.block
The PLATELET.BLOCK file denotes "block" against the cut positions. PLASMIDMAP interprets these accordingly. We can use "block", "range" and "tick" features together if we edit the file.
In chapter 3 we used the "tick" file produced by MAPSORT/PLASMID, it contained information for PLASMIDMAP to mark the enzymes cut sites:
$Mapsort/mincuts=2/maxcuts=2/plasmid/Infile=platelet.seq
{accept all the defaults}
Now edit the platelet.tick file to create a label for the region between the two HaeI sites.
HaeI 844 844 . Black . .
Tick
HaeI 972 972 . Black . .
Tick
MaeII 724 724 . Green . .
Tick
MaeII 857 857 . Green . .
Tick
MaeIII 1091 1091 . Green . .
Tick
MaeIII 1215 1215 . Green . .
Tick
To read:
HaeI 844 972 + Black | >
Range
MaeII 724 857 - Green > >
Range
MaeIII 1091 1215 + Green . .
Block
The only styles available are tick, block and range. The direction of a range is controlled by a + or - in the strand column.
Display this in the usual way:
$Plasmidmap/font=1 platelet.tick
{accept all the defaults}