From <@VM.NRC.CA:Nash@biologysx.lan.nrc.ca> Thu Jan 30 14:49:02 1992 Received: from IUBIO.BIO.INDIANA.EDU by sunflower.bio.indiana.edu (4.1/9.5jsm) id AA00498; Thu, 30 Jan 92 14:48:59 EST From: Subject: DNAfrag 3.03 (IBM-PC) To: Date: Thu, 30 Jan 92 14:41:00 PST Message-Id: <29887F15@coursmtp.nrc.ca> Encoding: 67 TEXT, 741 TEXT X-Mailer: Network Courier V2.1b (beta-3) X-Nc-Attachment: DFRAG303.UUE 47112 01-30-1992 11:22 Status: R G'day, "bionet.software-sources" has been a bit quiet lately, so here's a little program (for the IBM-PC) that I thought folks may find useful... I have a couple more like it in the works. --- Short description: filename DNAfrag update 30 Jan 1992 version 3.03 size 47112 bytes compressed LHA self-extracting ASCII conversion UUE author John Nash description see long description below... literature - source code - sw requirements - hw requirements IBM PC, XT, AT, PS/2 or compatible with MDA, CGA, EGA, VGA or Hercules graphics card. --- Long description: DNAfrag version 3.03 This program is used in restriction mapping of DNA or sizing of proteins from agarose or SDS-PAGE gels. It calculates the size of restriction fragments or peptide bands on gels or autoradiograms if standards are run on the same gel. DNAfrag calculates a standard curve of the standard DNA or protein bands using their mobilities (measured by you) using the "Least squares fit of DNA fragment length to gel mobility" algorithm of Schaffer, H.E. and Sederoff, R.R. (1981), Analytical Biochemistry vol 115, pp 113-122. DNAFRAG was designed to be used in university "mass" computer lab facilities, and it should work on any sort of IBM PC, XT, AT, PS/2 or clone, using MDA, CGA, EGA, VGA or HGC video cards. A hard drive is NOT required and it will fit on a 360K 5.25" floppy disk. It was modified from a FORTRAN implementation derived from the original paper, and was re-written with a "user-friendly interface" for people who are unfamiliar with computers. I found it ESPECIALLY useful in a 4th year "Introduction to Molecular Genetics" lab class I taught (as have several colleagues). To run the program, type "DNAFRAG". It is completely menu driven, and contains an Instruction module. sincerely, John Dr John Nash, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada. Email: Nash@biologysx.lan.nrc.ca